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All Journal Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science AGROMIX Jurnal HPT (Hama Penyakit Tumbuhan)
Liliek Sulistyowati
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Agriculture, Biological Sciences & Forestry

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Journal : AGRIVITA, Journal of Agricultural Science

 1 
EXPRESSION OF RESISTANCE OF SOYBEAN TO THE POD SUCKING BUG RIPTORTUS LINEARIS F. (HEMIPTERA: COREIDAE) Suharsono Suharsono; Liliek Sulistyowati
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i1.137

Abstract

Factors involved in the mechanism of resistance of soybean to pod sucking bug R. linearis  were identified using resistant soybean genotypes, IAC-100, and IAC-80-596-2 and the susceptible variety, Wilis as a check. The role of trichomes in resistance was assayed removing trihomes from the pod shell, and seed coat  and  the resistance was determined based on the number of stylet punctures made by the bug. Seed of IAC-100 and IAC-596-2 that had longer, denser trichomes, higher crude fiber content and  suffered fewer stylet punctures than  Wilis. This suggested that denser and longer trichomes interfered with stylet piercing of the pod shell. When the trichomes of IAC-100 and IAC-596-2 were removed these genotypes were more susceptible to insect feeding. In further studies, replacement of IAC-100 and IAC-596-2 seed with seed of Wilis in the pods of resistant genotypes resulted less stylet punctures on the Wilis seed. It was concluded that denser and longer trichomes on pods along with harder pod shells acts as a physical barrier in antixenosis resistance of soybean to the pod sucking bug. Therefore, IAC-100, and IAC-596-2 genotypes have good potential for used as resistant parents in a soybean breeding program.     Keywords: resistance, pod characters, tri-chomes, antixenosis, pod stink bug
Abundance of Metioche Vittalicollis ( Orthoptera:Gryllidae) and Natural Enemies in A Rice Agroecosystem as Influenced by Weed Species Sri Karindah; Bagyo Yanuwiadi; Liliek Sulistyowati; Peter T. Green
AGRIVITA, Journal of Agricultural Science Vol 33, No 2 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i2.55

Abstract

Weeds in the rice fields impact the population of M. vittaticollis and several predators on rice habitat. A study was conducted during rainy season 2004-2005 and dry season 2005, which selective weeding and weed strips were applied. In selective weeding, Monochoria vaginalis, Fimbristylis miliacea, Cyperus iria, and Limnocharis flava were left, and made a weed strip at the periphery of rice plot which was consisted of those four species. Clean weeding as farmers usually do was also applied as a control treatment. Results showed that the presence of weed plant species in the earlier planting season of dry season 2005 tended to maintain the population of the predatory insects of rice pests, in particularly M. vittaticollis and Anaxipha longipennis. Population of Ophionea nigrofasciata, Paederus spp. and Micraspis inops were not different among the treatment plots during rainy season (2004-2005) and dry season (2005). The species number of predator and parasitoids seemed to increase slower in the clean weeded plot in comparing with the species number in selectively weeded or weeds strips plot at early planting season. Rice yield in the experimental plots were not significantly different. Some weeds in the rice fields may not have adverse effects on rice productivity. Keywords : generalist predator, weeding, predatory cricket
Study of Expression of Sugarcane Sucrose Transporter cDNA in Yeast Slameto Slameto; Bambang Sugiharto; Nur Basuki; Liliek Sulistyowati
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.72

Abstract

Studies in transgenic sugarcane (Saccharum officinarum L.) demonstrated that sucrose transporter (SUT) genes were essential in sucrose translocations. Sucrose transporter gene isolated from sugarcane were designated as SoSUT1 and SoSOSUT2 respectively as encode protein of 518 and 747 amino acids. The genes were constructed into plasmid pYES2 for SoSUT1, and pYX112 for SoSOSUT2. cDNA SoSOSUT2 had also constructed into plasmid pBIN-At-GFP which was possible to trace the gene inserted. The constructed plasmid was transformed into yeast (Saccharomyces cerevisiae) and grown in minimal medium (SD–urasil) as selection medium. The transformed colony was confirmed using PCR. The functional expression was studied by growing yeast in YPD medium with 2% Sucrose, then the sucrose uptake was measured in number interval time using resorcinol method. The result showed that yeast INVSc1-pYES2-SoSUT1 and BF264- pYX112-SoSOSUT2 had higher ability in sucrose transport compared to the control-INVSc1 for SoSUT1 and control-BF264 for SoUT2. Moreover, the result showed that SoSUT1 had higher ability to transport sucrose than SoSOSUT2. Confocal microscope observation showed that transformation gene SoSOSUT2 was successful, which was indicated by green exposure of GFP protein.Keyword: sugarcane, sucrose transporter, yeast
Co-Authors Abdul Cholil Anton Muhibbudin Anton Muhibuddin Ari Kristini Bagyo Yanuwiadi Bambang Sugiharto Birtha Niken Pratiwi DIAN WULANDARI Dinnar Kusumaningtyas Erviani Marlitasari Esti Dwi Rahayu Fery Abdul Choliq Mintarto Martosudiro Nur Basuki Nurul Umayatul Aliah Peter T. Green Restu Rizkyta Kusuma Rosy Husna Shofiana Slameto Slameto Soleudin Efendi Sri Karindah Suharsono Suharsono Syamsuddin Djauhari Tsang Hai Chen Yunita Dian Puspita Yuricha Kusumawardani